Detection of interaction between an RNA aptamer and its target compound in living human cells using 2D in-cell NMR
Omar Eladl, Yudai Yamaoki, Keiko Kondo, Takashi Nagata, Masato Katahira
Abstract
transcription into HeLa cells. We successfully recorded the first heteronuclear 2D in-cell NMR spectra, which makes it possible to study the interaction of the RNA aptamer with argininamide in living human cells with higher resolution.
Topics & Concepts
AptamerRNAChemistryCellCombinatorial chemistryComputational biologyBiophysicsBiochemistryBiologyMolecular biologyGeneAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanismsDNA and Nucleic Acid Chemistry