Serum miR‐224, miR‐760, miR‐483‐5p, miR‐378 and miR‐375 as potential novel biomarkers in rheumatoid arthritis
Omayma O. Abdelaleem, Nermeen Ahmed Fouad, Olfat Shaker, Tarek I. Ahmed, Noha K. Abdelghaffar, Hanaa M. Eid, Abdelrahmaan A. Mohamed, Ahmed Elebiary, Mohamed Mokhtar Mohamed, Rania H. Mahmoud
Abstract
BACKGROUND: Rheumatoid arthritis (RA) is an inflammatory autoimmune disease which affects various tissues and organs mainly joints. Serum microRNAs are considered a new class of non-coding RNA which plays a vital role in pathogenesis of RA. METHODS: The current study was conducted on 80 RA patients and 80 healthy participants. Serum expression levels of miR-224, miR-760, miR-483-5p, miR-378 and miR-375 were evaluated via real-time quantitative polymerase chain reaction (PCR). RESULTS: Significant upregulation of miR-224, miR-760, miR-483-5p, miR-378 and miR-375 was reported in the present study with respect to the control group (P = .031, P = .017, P = .026, P = .036 and P = .05, respectively). Furthermore, significant positive correlation between the abovementioned microRNAs with DAS28 score (P < .001, each) was demonstrated. CONCLUSION: Early detection of RA could be achieved through evaluation of serum expression of miR-224, miR-760, miR-483-5p, miR-378 and miR-375 which also may be used as targets for treatment of patients with RA.