AAV-mediated delivery of a Sleeping Beauty transposon and an mRNA-encoded transposase for the engineering of therapeutic immune cells
Lupeng Ye, Stanley Z. Lam, Luojia Yang, Kazushi Suzuki, Yongji Zou, Qianqian Lin, Yueqi Zhang, Paul Clark, Lei Peng, Sidi Chen
Topics & Concepts
TransposaseTransgeneBiologySleeping Beauty transposon systemChimeric antigen receptorElectroporationGene deliveryGenetic enhancementInsertional mutagenesisCell biologyTransduction (biophysics)Molecular biologyTransposable elementImmune systemGeneImmunologyImmunotherapyGeneticsGenomeBiochemistryVirus-based gene therapy researchCAR-T cell therapy researchCRISPR and Genetic Engineering