Phytofabrication of copper nanoparticles using Momordica cymbalaria fruit extract and their antibacterial, antioxidant and anti-inflammatory properties
Shazia Parveen, Khateef Riazunnisa
Abstract
Copper nanoparticles, or CuNPs, are utilized across various scientific, technological, and engineering domains due to their nanoscale dimensions. The objective of this investigation is to synthesize eco-friendly nanoparticles using Momordica cymbalaria fruit and evaluate their biomedical applications, emphasizing their antibacterial, antioxidant, and anti-inflammatory characteristics. SEM analysis revealed that the produced copper nanoparticles were spherical in shape. Using Debye–Scherer equation, the average size of the green synthesized MC-CuNPs was determined to be approximately 14 nm. FTIR analysis revealed the functional groups of biomolecules involved in the stability and bioreduction of MC-CuNPs. The highest zone of inhibition was observed with Proteus vulgaris (23.1 ± 0.8 mm) and Staphylococcus aureus (23.3 ± 2.0 mm) compared to Escherichia coli and Salmonella typhimurium with 100 μg/ml MC-CuNPs. MC-CuNPs exhibited significant potential in scavenging free radicals and H 2 O 2. The IC 50 values of MC-CuNPs for total antioxidant and H 2 O 2 scavenging activities were 100 and 50 μg/ml respectively. The anti-inflammatory properties were evaluated against egg albumin's protein denaturation, with the highest level of protein inhibition recorded at 500 μg/ml of MC-CuNPs. Therefore, the synthesized nanoparticles may be utilized for biomedical applications.