Inactivation of Nitrite-Dependent Nitric Oxide Biosynthesis Is Responsible for Overlapped Antibiotic Resistance between Naturally and Artificially Evolved Pseudomonas aeruginosa
Su-fang Kuang, Ding-Yun Feng, Zhuang‐Gui Chen, Zhuozheng Liang, Juanjuan Xiang, Hui Li, Xuan‐xian Peng, Tiantuo Zhang
Abstract
. This is because the pyruvate cycle fluctuates, thereby generating fewer NADH and providing fewer electrons for nitrite-dependent NO biosynthesis than the control. Interestingly, exogenous fumarate, NADH, nitrate, and nitrite as well as NO donor sodium nitroprusside promote NO generation to elevate sensitivity to SCF. These results highlight the way to understand metabolic mechanisms of antibiotic resistance and explore metabolic modulation to combat the bacterial pathogen.
Topics & Concepts
NitritePseudomonas aeruginosaBiochemistryNitric oxideMicrobiologyCitric acid cycleBiosynthesisMetabolomeChemistryMetabolismNitrite reductaseNitrateBiologyBacteriaEnzymeMetaboliteGeneticsOrganic chemistryAntibiotic Resistance in BacteriaPharmaceutical and Antibiotic Environmental ImpactsCystic Fibrosis Research Advances