Structural insight into the cGAS active site explains differences between therapeutically relevant species
Alexander Skeldon, Li Wang, Nicolas Sgarioto, Ramsay E. Beveridge, S. Y. T. Chan, Stéphane Dorich, Valérie Dumais, Nadine Fradet, Samuel Gaudreault, Philippe LeGros, D.B. McKay, Ria Seliniotakis, Daniel V. Sietsema, Lingling Zhang, Marc-Olivier Boily, Jason D. Burch, Alexandre Caron, Lee D. Fader, Lodoe Lama, Wei Xie, Dinshaw J. Patel, Thomas Tuschl, Michael A. Crackower, Kelly A. Pike
Abstract
Cyclic GMP-AMP synthase (cGAS) is an intracellular sensor of double-stranded DNA that triggers a pro-inflammatory response upon binding. The interest in cGAS as a drug discovery target has increased substantially over the past decade due to growing evidence linking its activation to numerous peripheral and neurological diseases. Here, we report the binding mode of previously described cGAS inhibitors while also uncovering the structural basis for the interspecies potency shifts within this chemotype. A single threonine to isoleucine substitution between human and mouse cGAS drives compound activity, as demonstrated by biochemical, cellular, and in vivo studies. Finally, we utilize a structurally enabled design approach to engineer a novel chemical inhibitor with excellent potency for both human and mouse enzymes by targeting key interactions within the enzyme active site. Overall, this work provides the framework for rational optimization of cGAS inhibitors and potential preclinical translational strategies. Cyclic GMP-AMP synthase (cGAS), an intracellular sensor of dsDNA, is a central driver of inflammatory diseases and an emerging therapeutic target, although optimization of cGAS inhibitors for the clinic is challenging because of the incomplete understanding of their mode of action. Here, the authors study the binding mode of G-chemotype human cGAS inhibitors, revealing key structural differences driving cGAS inhibitor cross-species potency shifts, and discover a novel cGAS inhibitor with exceptional mouse and human potency.