Use of EpiAlveolar Lung Model to Predict Fibrotic Potential of Multiwalled Carbon Nanotubes
Hana Barošová, Anna G. Maione, Dedy Septiadi, Monita Sharma, Laetitia Haeni, Sandor Balog, Olivia O’Connell, George R. Jackson, David M. Brown, Amy J. Clippinger, Patrick Hayden, Alke Petri‐Fink, Vicki Stone, Barbara Rothen‐Rutishauser
Abstract
) using an air-liquid interface exposure device (VITROCELL Cloud) with repeated exposures over 3 weeks. Specific key events leading to lung fibrosis, such as barrier integrity and release of proinflammatory and profibrotic markers, show the responsiveness of the model. Nanocyl induced, in general, a less pronounced reaction than Mitsui-7, and the cultures with human monocyte-derived macrophages (MDMs) showed the proinflammatory response at later time points than those without MDMs. In conclusion, we present a robust alveolar model to predict inflammatory and fibrotic responses upon exposure to MWCNTs.