Production of Cross-Linked Lipase Crystals at a Preparative Scale
Raquel Fernández-Penas, Cristóbal Verdugo‐Escamilla, Sergio Martínez‐Rodríguez, José A. Gavira
Abstract
-nitrophenol butyrate and α-methylbenzyl acetate, respectively. RCLLCs presented both high thermal and robust operational stability, allowing the preparation of a packed-bed chromatographic column to work in a continuous flow. Finally, we determined the three-dimensional (3D) models of this commercial lipase crystallized with and without phosphate at 2.0 and 1.7 Å resolutions, respectively.
Topics & Concepts
CrystallizationLipaseDownstream processingChromatographyAgaroseChemistryExtraction (chemistry)Aqueous solutionHexaneSolubilitySolventRaw materialProtein crystallizationChemical engineeringMaterials scienceOrganic chemistryEnzymeEngineeringEnzyme Catalysis and ImmobilizationEnzyme Structure and FunctionMicrobial Metabolic Engineering and Bioproduction