Enzymatically produced piggyBac transposon vectors for efficient non-viral manufacturing of CD19-specific CAR T cells
Iva Kaštánková, Martin Štach, Hana Žižková, Pavlína Ptáčková, Kristýna Šmilauerová, Martin Mucha, Vojtěch Šroller, Pavel Otáhal
Abstract
via enzymatic synthesis. It is suitable for the cost-effective production of highly experimental, early-phase CAR T cell products.
Topics & Concepts
TransposaseBiologyTransposable elementElectroporationMolecular biologyVirologyGeneGeneticsGenomeCAR-T cell therapy researchViral Infectious Diseases and Gene Expression in InsectsVirus-based gene therapy research