In-Depth Profiling of <i>O</i>-Glycan Isomers in Human Cells Using C18 Nanoliquid Chromatography–Mass Spectrometry and Glycogenomics
Noortje de Haan, Yoshiki Narimatsu, Mikkel Koed Møller Aasted, Ida Signe Bohse Larsen, Irina N. Marinova, Sally Dabelsteen, Sergey Y. Vakhrushev, Hans H. Wandall
Abstract
-GalNAc glycosylation, with the latter carrying both elongated core1 and core2 structures and varying numbers of fucoses and sialic acids. The method, including the now well-characterized standards, provides the opportunity to study glycomic changes in human tissue and disease models using rather mainstream analytical equipment.
Topics & Concepts
GlycanChemistryGlycomicsGlycosylationGlycomeMass spectrometryFucoseCleavage (geology)BiochemistryChromatographyComputational biologyGlycoproteinBiologyEngineeringGeotechnical engineeringFracture (geology)Glycosylation and Glycoproteins ResearchCarbohydrate Chemistry and SynthesisGenomics and Phylogenetic Studies