Extraction-free, one-pot CRISPR/Cas12a detection of microRNAs directly from extracellular vesicles
Yan He, Song Han, Steven J. Hughes, Yong Zeng
Abstract
Current methods for extracellular vesicle (EV) miRNA analysis mostly require RNA extraction, which results in a multi-step, time-consuming workflow. This study reports an extraction-free method that combines thermolysis treatment of EVs with a one-pot EXTRA-CRISPR assay, enabling the vastly simplified analysis of EV miRNAs with a comparable performance to that of the extraction-based assays.
Topics & Concepts
Extracellular vesiclesExtraction (chemistry)CRISPRRNA extractionmicroRNARNAChemistryChromatographyExtracellular vesicleComputational biologyVesicleExtracellularNucleic acidMicrovesiclesBiologyCell biologyBiochemistryGeneMembraneExtracellular vesicles in diseaseAdvanced biosensing and bioanalysis techniquesMicroRNA in disease regulation