Using a High Quantum Yield Fluorescent Probe with Two-Photon Excitation to Detect Cisplatin in Biological Systems
Fan Fang, Li Zhang, Fang-Ya Mu, Guoyue Shi
Abstract
A direct detection protocol for the anticancer drug of cisplatin is highly desirable for studying its actions and side effects. In this work, a high quantum yield fluorescent probe with two-photon excitation to detect cisplatin was designed. The probe (RD640-TC) was based on the rhodamine 640 (Rh640) fluorophore, responding to cisplatin with red fluorescence. It showed an excellent linear correlation between the fluorescence response and the concentration of cisplatin over the range of 2-50 μM, suggesting a feasible tool for convenient detection of cisplatin. RD640-TC had high fluorescence quantum yield (Φ = 0.68) and two-photon absorption properties, which made it more favorable to probe cisplatin in biological systems. We exemplified RD640-TC for the detection of cisplatin in cells and zebrafish, providing an accessible tool for in vivo tracking of cisplatin, which has great potential value for studying how cisplatin is processed at cellular level and further for facilitating the investigation into the origin of cisplatin's toxicity.