Lipid and polymer mediated CRISPR/Cas9 gene editing
Yan Gong, Siyu Tian, Xuan Yang, Shubiao Zhang
Abstract
A clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein 9 (CRISPR/Cas9) system is the most widely used tool for gene editing. Due to the advantages of simple operation, high efficiency and high specificity, it has been used in various fields. In this review, we introduce the latest research in the delivery technology of CRISPR/Cas9 based on lipids and polymers, which are synthesized through chemical methods. Many successful examples and methods are discussed. In addition, we discuss the potential of these delivery systems used for CRISPR/Cas9 in clinical applications in the future.
Topics & Concepts
CRISPRGenome editingCas9GenePolymerGeneticsComputational biologyBiologyChemistryOrganic chemistryCRISPR and Genetic EngineeringViral Infectious Diseases and Gene Expression in InsectsGenetics, Aging, and Longevity in Model Organisms