An Experimental Study on [ <sup>125</sup> I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment
Ming Yu, Yanqin Sun, Guangjie Yang, Zhenguang Wang
Abstract
Objective. To evaluate the diagnostic efficacy of MDA-MB-231 triple-negative breast cancer with 125 I-labeled pHLIP (Var7) by single-photon emission computed tomography/computed tomography (SPECT/CT) imaging. Methods. The binding fraction of [ 125 I]I-pHLIP (Var7) and MDA-MB-231 cells was measured at pH 7.4 and pH 6.0, and tumor-bearing mice were subjected to small-animal SPECT/CT imaging studies. Results. At [Formula: see text], the binding fractions of [ 125 I]I-pHLIP (Var7) and MDA-MB-231 cells at 10 min, 40 min, 1 h, and 2 h were 1[Formula: see text]%, [Formula: see text]%, [Formula: see text]%, and [Formula: see text]%, respectively. At [Formula: see text], there was no measured binding between [ 125 I]I-pHLIP (Var7) and MDA-MB-231 cells. Small-animal SPECT/CT imaging showed clearly visible tumors at 1 and 2 h after injection. Conclusions. [ 125 I]I-pHLIP (Var7) could bind to MDA-MB-231 cells in an acidic environment, and small-animal SPECT/CT imaging showed clear tumors at 1 and 2 h after probe injection.