Engineering fluorescent protein chromophores with an internal reference for high-fidelity ratiometric G4 imaging in living cells
Jiao‐Na Han, Zhong Caijun, Mingmin Ge, Shi Kuang, Zhou Nie
Abstract
tracking G4s. To the best of our knowledge, NHCouI is the first small molecule G4 probe enabled by internal reference correction capability, opening up new avenues for dual-emission chromophore development and high-fidelity and reliable analysis in G4 imaging research.
Topics & Concepts
ChromophoreFluorescenceChemistryQuantum yieldStokes shiftGreen fluorescent proteinBiophysicsPhotochemistryPhysicsBiochemistryOpticsBiologyGeneAdvanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryRNA Interference and Gene Delivery