Direct Nanopore Sequencing of Individual Full Length tRNA Strands
Niki K. Thomas, Vinay Chaitanya Poodari, Miten Jain, Hugh E. Olsen, Mark Akeson, Robin Abu-Shumays
Abstract
tRNA alignments, including a highly conserved pseudouridine in the T loop. This work highlights the potential of nanopore direct tRNA sequencing as well as improvements needed to implement tRNA sequencing for human healthcare applications.
Topics & Concepts
MinionTransfer RNANanopore sequencingNanoporePseudouridineComputational biologyBiologyRNAGeneticsDNA sequencingGeneNanotechnologyMaterials scienceRNA modifications and cancerRNA and protein synthesis mechanismsGenomics and Phylogenetic Studies