Litcius/Paper detail

Droplet-based methods for tackling antimicrobial resistance

Artur Ruszczak, Simona Bartkova, Marta Zapotoczna, Ott Scheler, Piotr Garstecki

2022Current Opinion in Biotechnology19 citationsDOIOpen Access PDF

Abstract

Application of droplet-based methods enables (i) faster detection, (ii) increased sensitivity, (iii) characterization of the level of heterogeneity in response to antibiotics by bacterial populations, and (iv) expanded screening of the effectiveness of antibiotic combinations. Hereby, we discuss the key steps and parameters of droplet-based experiments to investigate antimicrobial resistance. We also review recent findings accomplished with these methods and highlight their advantages and capacity to yield new insights into the problem of antimicrobial resistance.

Topics & Concepts

AntimicrobialAntibiotic resistanceAntibioticsBiochemical engineeringSensitivity (control systems)Yield (engineering)Key (lock)NanotechnologyBiotechnologyBiologyBiological systemComputer scienceMicrobiologyComputational biologyMaterials scienceEngineeringEcologyMetallurgyElectronic engineeringBacterial Identification and Susceptibility TestingInnovative Microfluidic and Catalytic Techniques InnovationCell Image Analysis Techniques