Biocompatible Biogenic Silver Nanoparticles Interact with Caspases on An Atomic Level to Elicit Apoptosis
Shalini Kumari, Puja Kumari, Pritam Kumar Panda, Paritosh Patel, Ealisha Jha, M. Anwar Mallick, Mrutyunjay Suar &, Suresh K. Verma
Abstract
Aim: To synthesize biocompatible and ecofriendly silver nanoparticles (AgNPs) and elucidate their in vivo molecular and cellular nanotoxicity at an atomic level. Materials & methods: AgNPs were biosynthesized using Andrographolide and their in vivo cellular and molecular biocompatibility was evaluated using zebrafish embryos. Results: AgNPs with a size of 80 nm and ζ potential of -52 mV were obtained. The LC50 for the AgNPs embryonic zebrafish was found to be 125 μg/ml. AgNPs induced reactive oxygen species production and elicit apoptosis mechanistically, then nanoparticles were shown to interact with caspase-3 and caspase-9 proteins through proline, cysteine, glycine and histidine amino acid residues via H-bond of corresponding bond energies. Conclusion: Biosynthesized AgNPs have potential to be used for biomedical and therapeutic applications.