Rutin-Loaded Liquid Crystalline Nanoparticles Attenuate Oxidative Stress in Bronchial Epithelial Cells: A PCR Validation
Meenu Mehta, Keshav Raj Paudel, Shakti D. Shukla, Madhur D. Shastri, Saurabh Satija, Sachin Kumar Singh, Monica Gulati, Harish Dureja, Flavia C. Zacconi, Philip M. Hansbro, Dinesh Kumar Chellappan, Kamal Dua
Abstract
Aim: In the present study, the inhibitory potential of rutin-loaded liquid crystalline nanoparticles (LCNs) on oxidative stress was determined in human bronchial epithelial cells (BEAS-2B) by analysing the expression levels of different antioxidant (NADPH quinine oxidoreductase-1 (NQO1); γ-glutamyl cysteine synthetase catalytic subunit (GCLC)) and pro-oxidant (NADPH oxidase (Nox)-4; Nox2B) genes. Results: Our findings revealed that the rutin-loaded LCNs inhibited the genes, namely Nox2B and Nox4, which caused oxidative stress. In addition, these nanoparticles demonstrated an upregulation in the expression of the antioxidant genes Gclc and Nqo-1 in a dose-dependent manner. Conclusion: The study indicates the promising potential of rutin-loaded LCNs as an effective treatment strategy in patients with high oxidant loads in various respiratory diseases.