Miniature Multiwavelength Deep UV-LED-Based Absorption Detection System for Capillary LC
Mohamed Hemida, Lewellwyn J. Coates, Shing Chung Lam, Vipul Gupta, Mirek Macka, Hans‐Jürgen Wirth, Andrew A. Gooley, Paul R. Haddad, Brett Paull
Abstract
A new miniature deep UV absorbance detector has been developed using low-cost and high-performance LEDs, which can be operated in both scanning (230 to 300 nm) and individual wavelength (240, 255, and 275 nm) detection modes. The detector is mostly composed of off-the-shelf components, such as LEDs, trifurcated fiber optic assembly, a capillary Z-type flow cell, and photodiodes. It has been characterized for use with a standard capillary LC system and was benchmarked against a standard variable wavelength capillary LC detector. The detector shows very low levels of stray light (<0.4%), utilization of up to 99.0% of the effective path length of the flow cell, a wide dynamic range (0.5 to 200 μg/mL for sulfamethazine, carbamazepine, and flavone), and low noise levels (at 300 μAU level). The detector was applied within a miniaturized LC system.