Dual Cre and Dre recombinases mediate synchronized lineage tracing and cell subset ablation in vivo
Haixiao Wang, Lingjuan He, Yan Li, Wenjuan Pu, Shaohua Zhang, Ximeng Han, Kathy O. Lui, Bin Zhou
Abstract
cells after diphtheria toxin administration. These mouse lines were used to simultaneously trace and deplete the target cells of interest through the inducible expression of a reporter and DTR using dual Cre and Dre recombinases, allowing a more precise and efficient study of the role of specific cell subsets within a heterogeneous population in pathophysiological conditions in vivo.
Topics & Concepts
RecombinaseCre recombinaseBiologyDiphtheria toxinFate mappingCell biologyCre-Lox recombinationPopulationTransgeneChimera (genetics)Reporter geneGeneticsGeneMolecular biologyGenetically modified mouseStem cellGene expressionProgenitor cellRecombinationDemographyToxinSociologyCRISPR and Genetic EngineeringViral Infectious Diseases and Gene Expression in InsectsCell Image Analysis Techniques