<i>MIR‐138‐5P</i> inhibits the progression of prostate cancer by targeting <i>FOXC1</i>
Hui Huang, Ying Xiong, Zhensheng Wu, Yuhui He, Xianglin Gao, Zhangyan Zhou, Tao Wang
Abstract
BACKGROUND: Studies have suggested that micro-RNAs (miRNAs) can function as an oncogene or a tumor suppressor in cancers. However, the role of MIR-138-5P (613394) in prostate cancer (PCa) remains unclear. METHODS: Expression level of MIR-138-5P in PCa cell lines and normal cell line was analyzed with the quantitative real-time PCR method. Cell counting kit-8 assay, colony formation assay, wound-healing assay, and transwell invasion assay were performed to analyze the biological functions of MIR-138-5P. RESULTS: We showed MIR-138-5P expression level was significantly decreased in PCa cell lines compared with the normal cell line. Overexpression of MIR-138-5P inhibits PCa cell proliferation, colony formation, cell migration, and cell invasion in vitro. Mechanistically, we showed Forkhead box C1 (FOXC1, 601090) was a direct target for MIR-138-5P in PCa. We confirmed that overexpression of FOXC1 partially reversed the effects of MIR-138-5P on PCa cell behaviors. CONCLUSIONS: Collectively, we showed that MIR-138-5P functions as a tumor suppressor gene in PCa via targeting FOXC1.