Instrumental analysis of RNA modifications
Yasemin Yoluç, Gregor Ammann, Pierre Barraud, Manasses Jora, Patrick A. Limbach, Yuri Motorin, Virginie Marchand, Carine Tisné, Kayla Borland, Stefanie Kellner
Abstract
Organisms from all domains of life invest a substantial amount of energy for the introduction of RNA modifications into nearly all transcripts studied to date. Instrumental analysis of RNA can focus on the modified residues and reveal the function of these epitranscriptomic marks. Here, we will review recent advances and breakthroughs achieved by NMR spectroscopy, sequencing, and mass spectrometry of the epitranscriptome.
Topics & Concepts
RNABiologyMass spectrometryNucleic acid structureComputational biologyGeneticsChemistryGeneChromatographyRNA modifications and cancerRNA and protein synthesis mechanismsCancer-related molecular mechanisms research