Toxicological Responses of α-Pinene-Derived Secondary Organic Aerosol and Its Molecular Tracers in Human Lung Cell Lines
Faria Khan, Karina Kwapiszewska, Yue Zhang, Yuzhi Chen, Andrew T. Lambe, Agata Kołodziejczyk, Nasir Jalal, Krzysztof J. Rudziński, Alicia Martínez‐Romero, Rebecca C. Fry, Jason D. Surratt, Rafał Szmigielski
Abstract
at 24 and 48 h, respectively. An approximate 4-fold increase in cellular oxidative stress was observed in BEAS-2B cells when compared with untreated cells, suggesting that reactive oxygen species (ROS) buildup resulted in the downstream cytotoxicity following 24 h of exposure to α-pinene SOA. Organic hydroperoxides that were identified in the α-pinene SOA samples likely contributed to the ROS and cytotoxicity. This study identifies the potential components of α-pinene SOA that likely modulate the oxidative stress response within lung cells and highlights the need to carry out chronic exposure studies on α-pinene SOA to elucidate its long-term inhalation exposure effects.