Characterization of antibody-antigen interactions using biolayer interferometry
Tal Noy-Porat, Ron Alcalay, Adva Mechaly, Eldar Peretz, Efi Makdasi, Ronit Rosenfeld, Ohad Mazor
Abstract
This protocol describes the use of a biolayer interferometry platform for assessing antibody-antigen interactions. The protocol focuses on affinity determination and epitope binning, although the system can be utilized for measuring any protein-protein interaction. Readings are collected in real time, allowing the use of unlabeled molecules, and data can thus be obtained in a fast and easy manner. Experiments should be carefully designed, taking into consideration the tested interaction, available sensors, and suitable controls. For complete details on the use and execution of this protocol, please refer to Noy-Porat et al. (2021).
Topics & Concepts
Protocol (science)EpitopeComputer scienceAntigenAntibodyComputational biologyInterferometryChemistryMedicineImmunologyBiologyPhysicsOpticsPathologyAlternative medicineMonoclonal and Polyclonal Antibodies ResearchAdvanced Biosensing Techniques and ApplicationsViral Infectious Diseases and Gene Expression in Insects