Between life and death: strategies to reduce phototoxicity in super-resolution microscopy
Kalina L. Tosheva, Yue Yuan, Pedro M. Pereira, S J Culley, Ricardo Henriques
Abstract
Super-resolution microscopy (SRM) enables non-invasive, molecule-specific imaging of the internal structure and dynamics of cells with sub-diffraction limit spatial resolution. One of its major limitations is the requirement for high-intensity illumination, generating considerable cellular phototoxicity. This factor considerably limits the capacity for live-cell observations, particularly for extended periods of time. Here, we give an overview of new developments in hardware, software and probe chemistry aiming to reduce phototoxicity. Additionally, we discuss how the choice of biological model and sample environment impacts the capacity for live-cell observations.
Topics & Concepts
PhototoxicityResolution (logic)MicroscopyDiffractionSuperresolutionLive cell imagingImage resolutionNanotechnologyBiological systemComputer scienceOpticsMaterials scienceChemistryCellPhysicsBiologyArtificial intelligenceImage (mathematics)In vitroBiochemistryAdvanced Fluorescence Microscopy TechniquesCell Image Analysis TechniquesAdvanced Electron Microscopy Techniques and Applications