<i>In situ</i> detection of plasma exosomal microRNA for lung cancer diagnosis using duplex-specific nuclease and MoS<sub>2</sub> nanosheets
Zibo Gao, Huijie Yuan, Yanhua Mao, Lihua Ding, Clement Yaw Effah, Sitian He, Leiliang He, Lie Liu, Songcheng Yu, Yilin Wang, Jia Wang, Yongmei Tian, Fei Yu, Hongchao Guo, Lijun Miao, Lingbo Qu, Yongjun Wu
Abstract
was used to selectively quench the fluorescence of the undigested probe leaving the fluorescent signal of the fluorescently-labeled probe fragments. The fluorometric signals for miRNA-21 had a maximum excitation/emission wavelength of 488/518 nm. Most importantly, the biosensor was then applied for the accurate quantitative detection of miRNA-21 in exosome lysates extracted from human plasma and this method was able to successfully distinguish lung cancer patients from healthy people. This biosensor provides a simple, rapid, and a highly specific quantitative method for exosomal miRNA and has promising potential to be used in the early diagnosis of lung cancer.