Comparison of nine different commercially available molecular assays for detection of SARS-CoV-2 RNA
Ute Eberle, Clara Wimmer, Ingrid Huber, Antonie Neubauer‐Juric, Giuseppe Valenza, Nikolaus Ackermann, Andreas Sing
Abstract
To face the COVID-19 pandemic, the need for fast and reliable diagnostic assays for the detection of SARS-CoV-2 is immense. We describe our laboratory experiences evaluating nine commercially available real-time RT-PCR assays. We found that assays differed considerably in performance and validation before routine use is mandatory.
Topics & Concepts
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)Virology2019-20 coronavirus outbreakMedical microbiologyPandemicMolecular diagnosticsMedicineBiologyComputational biologyPathologyBioinformaticsInfectious disease (medical specialty)OutbreakDiseaseSARS-CoV-2 detection and testingSARS-CoV-2 and COVID-19 ResearchBiosensors and Analytical Detection