Single-Cell Transcriptome Sequencing and Proteomics Reveal Neonatal Ileum Dynamic Developmental Potentials
Qingshi Meng, Liang Chen, Bohui Xiong, Beining Kang, Pengfei Zhang, Shanlong Tang, Hui Han, Wei Shen, Xiaohui Feng, Shengnan Feng, Ruqing Zhong, Xiangfang Tang, Sheng Zhang, Hongfu Zhang, Yong Zhao
Abstract
We found previously unknown neonatal ileum developmental potentials: specific increases in undifferentiated cells, unique enterocyte differentiation, and time dependent reduction in secretory cells. Specific transcriptional factors (TFs), ligand-receptor pairs, G protein-coupled receptors, transforming growth factor β, bone morphogenetic protein signaling pathways, and the gut mucosal microbiota are involved in this process. Our results may assist in the creation of novel interventions for inflammation-, metabolism-, and proliferation-related gut pathologies.