Low-Level Rifampin Resistance and <i>rpoB</i> Mutations in Mycobacterium tuberculosis: an Analysis of Whole-Genome Sequencing and Drug Susceptibility Test Data in New York
Joseph Shea, Tanya A. Halse, Donna Kohlerschmidt, Pascal Lapierre, Herns Modestil, Cheryl Kearns, Felicia Dworkin, Jennifer L. Rakeman, Vincent Escuyer, Kimberlee A. Musser
Abstract
Rapid and reliable detection of rifampin (RIF) resistance is critical for the diagnosis and treatment of drug-resistant and multidrug-resistant (MDR) tuberculosis. Discordant RIF phenotype/genotype susceptibility results remain a challenge due to the presence of rpoB mutations that do not confer high levels of RIF resistance, as have been exhibited in strains with mutations such as Ser450Leu.
Topics & Concepts
rpoBMycobacterium tuberculosisDrug resistanceWhole genome sequencingBiologyGeneticsTuberculosisRifampicinGenomeVirologyAntibioticsMedicineGenePathologyTuberculosis Research and EpidemiologyMycobacterium research and diagnosisDiagnosis and treatment of tuberculosis