Stable isotope dilution mass spectrometry quantification of hydrogen sulfide and thiols in biological matrices
Hind Malaeb, Ibrahim Choucair, Zeneng Wang, Xinmin S. Li, Lin Li, W. Christopher Boyd, Christopher Hine, W.H. Wilson Tang, Valentin Gogonea, Stanley L. Hazen
Abstract
BACKGROUND: S research is limited by the lack of an accurate internal standard-containing assay for its quantitation in biological matrices. METHODS: S and thiols in plasma from 400 subjects, and within 20 volunteers before and after antibiotic-induced suppression of gut microbiota. RESULTS: S levels showed significant age-associated reductions in both males and females (p < 0.001), and a marked reduction following gut microbiota suppression (mean 33.8 ± 17.7%, p < 0.001), with large variations in gut microbiota contribution among subjects (range 6.0-66.7% reduction with antibiotics). CONCLUSIONS: S levels in humans.