Nuclear Phosphatidylinositol 3,4,5-Trisphosphate Interactome Uncovers an Enrichment in Nucleolar Proteins
Fatemeh Mazloumi Gavgani, Malene Skuseth Slinning, Andrea Papdiné Morovicz, Victoria Smith Arnesen, Diana C. Turcu, Sandra Ninzima, Clive S. D’Santos, Aurélia E. Lewis
Abstract
The polyphosphoinositide (PPIn) phosphatidylinositol 3,4,5 trisphosphate (PtdIns(3,4,5)P 3 ) localizes to the nucleus and nucleolus. Using an affinity enrichment MS approach, the nuclear PtdIns(3,4,5)P 3 interactome identified new interaction partners associated with the nucleolus. Among these, the DNA repair PARP1 protein, colocalized to the nucleolus with PtdIns(3,4,5)P 3 and showed direct interaction to PPIn via three polybasic regions. The nuclear PtdIns(3,4,5)P 3 interactome reported here will serve as a resource to further investigate the molecular mechanisms underlying PtdIns(3,4,5)P 3 -mediated interactions in the nucleus and nucleolus.