Cell culture‐derived extracellular vesicles: Considerations for reporting cell culturing parameters
Faezeh Shekari, Faisal J. Alibhai, Hossein Baharvand, Verena Börger, Stefania Bruno, Owen G. Davies, Bernd Giebel, Mario Gimona, Ghasem Hosseini Salekdeh, Lorena Martín‐Jaular, Suresh Mathivanan, Inge Nelissen, Esther N. M. Nolte‐‘t Hoen, Lorraine O’Driscoll, Francesca Perut, Stefano Pluchino, Gabriella Pòcsfalvi, Carlos Salomón, Carolina Soekmadji, Simon Staubach, Ana Cláudia Torrecilhas, Ganesh Vilas Shelke, Tobias Tertel, Dandan Zhu, Clotilde Théry, Kenneth W. Witwer, Rienk Nieuwland
Abstract
Cell culture-conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. Cell culturing parameters affect the biochemical composition, release and possibly the function of CCM-derived EVs (CCM-EV). The CCM-EV task force of the Rigor and Standardization Subcommittee of the International Society for Extracellular Vesicles aims to identify relevant cell culturing parameters, describe their effects based on current knowledge, recommend reporting parameters and identify outstanding questions. While some recommendations are valid for all cell types, cell-specific recommendations may need to be established for non-mammalian sources, such as bacteria, yeast and plant cells. Current progress towards these goals is summarized in this perspective paper, along with a checklist to facilitate transparent reporting of cell culturing parameters to improve the reproducibility of CCM-EV research.