Enhanced <i>O</i> ‐succinyl‐ <scp>l</scp> ‐homoserine production by recombinant <i>Escherichia coli</i> ΔIJBB*Trc <i>metL</i> /pTrc‐ <i>metA</i> <sup>fbr</sup> ‐Trc‐ <i>thrA</i> <sup>fbr</sup> ‐ <i>yjeH</i> via multilevel fermentation optimization
Wenyu Zhu, Kun Niu, Pi Liu, Y.‐H. Fan, Zhi‐Qiang Liu, Yu‐Guo Zheng
Abstract
AIMS: Constructing a strain with high yield of O-succinyl-l-homoserine (OSH) and improving the titre through multilevel fermentation optimization. METHODS AND RESULTS: ). CONCLUSIONS: O-succinyl-l-homoserine fermentation process was established and the combination of response surface methodology and metabolic pathway analysis effectively improved the titre of OSH. SIGNIFICANCE AND IMPACT OF THE STUDY: In this study, the titre of OSH reached the needs for industrial production and the metabolic pathway of OSH was demonstrated for further optimization.
Topics & Concepts
FermentationTiterStrain (injury)Response surface methodologyEscherichia coliRecombinant DNAThreonineBiochemistryBox–Behnken designYeast extractYeastIndustrial fermentationChemistryBiologyFood scienceChromatographyGeneEnzymeSerineGeneticsAntibodyAnatomyMicrobial Metabolic Engineering and BioproductionCRISPR and Genetic EngineeringViral Infectious Diseases and Gene Expression in Insects