Differentiation of Community-Associated and Livestock-Associated Methicillin-Resistant Staphylococcus aureus Isolates and Identification of <i>spa</i> Types by Use of PCR and High-Resolution Melt Curve Analysis
Seyed A. Ghorashi, Jane Heller, Quincy Zhang, Shafi Sahibzada
Abstract
Infections due to methicillin-resistant Staphylococcus aureus (MRSA) are present worldwide and represent a major public health concern. The capability of PCR followed by high-resolution melt (HRM) curve analysis for the detection of community-associated and livestock-associated MRSA strains and the identification of staphylococcal protein A ( spa ) locus was evaluated in 74 MRSA samples which were isolated from the environment, humans, and pigs on a single piggery. PCR-HRM curve analysis identified four spa types among MRSA samples and differentiated MRSA strains accordingly.
Topics & Concepts
Staphylococcus aureusMethicillin-resistant Staphylococcus aureusMicrobiologyHigh Resolution MeltLivestockBiologyLocus (genetics)Staphylococcal infectionsHigh resolutionPolymerase chain reactionBacteriaGeneGeneticsGeographyEcologyRemote sensingAntimicrobial Resistance in StaphylococcusBacterial Identification and Susceptibility TestingClostridium difficile and Clostridium perfringens research