A ratiometric pH probe for acidification tracking in dysfunctional mitochondria and tumour tissue<i>in vivo</i>
Yuming Zhang, Yuncong Chen, Hongbao Fang, Yanjun Wang, Shumeng Li, Hao Yuan, Shankun Yao, Shuheng Qin, Weijiang He, Zijian Guo
Abstract
upon the positively charged conjugated structure of hemicyanine fluorophore. Mitochondrial immobilization and acidification monitoring were realized by CouDa in cells treated with a mitochondrial uncoupler. Moreover, CouDa could distinguish acidified tumour tissue in living mice. Comparing with its analogue, the pH-sensitivity and mitochondria-immobilizing property are attributed to a hydrophobic long alkyl chain on indolium N atom. This work provides an effective strategy to track nucleophilic substances in dysfunctional mitochondria.
Topics & Concepts
In vivoDysfunctional familyMitochondrionBiophysicsCell biologyMaterials scienceNanotechnologyChemistryCancer researchBiologyMedicineGeneticsClinical psychologyATP Synthase and ATPases ResearchAnalytical Chemistry and SensorsEnzyme function and inhibition