Efficacy of Coxsackievirus A5 Vaccine Candidates in an Actively Immunized Mouse Model
Wei-Ping Jin, Jia Lu, Xiaoyu Zhang, Jie Wu, Zhenni Wei, Jianyi Mai, Shasha Qian, Yu‐Ting Yu, Shengli Meng, Zejun Wang, Shuo Shen
Abstract
The vaccine candidate strain CV-A5 was produced with a high infectivity titer and a high viral particle yield. Three particle forms, empty particles (EPs), full particles (FPs), and dense particles (DPs), were obtained and characterized after purification. The immunogenicities of EP, FP, and the EP and FP mixture were evaluated in mice. Mouse-adapted CV-A5 was generated as a challenge strain to infect 14-day-old mice. An active immunization challenge mouse model was established to evaluate the efficacy of the inactivated vaccine candidate. This animal model mimics vaccination, similar to immune responses of the vaccinated. The animal model also tests protective efficacy in response to the vaccine against the disease. This work is important for the preparation of multivalent vaccines against HFMD caused by different emerging strains.