Discovery and heterologous biosynthesis of glycosylated polyketide luteodienoside A reveals unprecedented glucinol-mediated product offloading by a fungal carnitine <i>O</i>-acyltransferase domain
Amr A. Arishi, Zhuo Shang, Ernest Lacey, Andrew Crombie, Daniel Vuong, Hang Li, Joe Bracegirdle, Peter Turner, William Lewis, Gavin R. Flematti, Andrew M. Piggott, Yit‐Heng Chooi
Abstract
-dimethylation of the 3-hydroxy-2,2,4-trimethylocta-4,6-dienoic acid intermediate, without requiring reversible product release and recapture by the cAT domain. This study expands the repertoire of polyketide modifications known to be catalysed by cAT domains and highlights the potential of mining fungal genomes for this subclass of fungal PKSs to discover new structurally diverse secondary metabolites.
Topics & Concepts
PolyketideAcyltransferaseBiosynthesisHeterologousChemistryDomain (mathematical analysis)Product (mathematics)BiochemistryNatural productComputational biologyBiologyEnzymeGeneMathematicsGeometryMathematical analysisMicrobial Natural Products and BiosynthesisCarbohydrate Chemistry and SynthesisFungal and yeast genetics research