A Robust Method for Perfusable Microvascular Network Formation In Vitro
Zhengpeng Wan, Amy X. Zhong, Shun Zhang, Georgios Pavlou, Mark F. Coughlin, Sarah E. Shelton, Huu Tuan Nguyen, Jochen H. Lorch, David A. Barbie, Roger D. Kamm
Abstract
Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two-step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established.
Topics & Concepts
SeedingMicrofluidicsBiomedical engineeringIn vitroComputer scienceChemistryMaterials scienceNanotechnologyBiologyMedicineBiochemistryAgronomy3D Printing in Biomedical ResearchInnovative Microfluidic and Catalytic Techniques InnovationElectrospun Nanofibers in Biomedical Applications