Uncovering the mechanisms of transcription elongation by eukaryotic RNA polymerases I, II, and III
Ruth Q. Jacobs, Zachariah I. Carter, Aaron L. Lucius, David A. Schneider
Abstract
transcription assays for direct comparison of their elongation rates, elongation complex (EC) stabilities, and fidelities. Pol I is the fastest, most likely to misincorporate, forms the least stable EC, and is most sensitive to alterations in reaction buffers. Pol II is the slowest of the Pols, forms the most stable EC, and negligibly misincorporated an incorrect nucleotide. The enzymatic properties of Pol III were intermediate between Pols I and II in all assays examined. These results reveal unique enzymatic characteristics of the Pols that provide new insights into their evolutionary divergence.
Topics & Concepts
RNA polymerase IITranscription (linguistics)PolymeraseElongationElongation factorRNA polymeraseRNABiologyEnzymeTranscription factor II DCell biologyGeneticsChemistryBiophysicsBiochemistryGene expressionGenePromoterMaterials scienceRibosomeUltimate tensile strengthPhilosophyMetallurgyLinguisticsGenomics and Chromatin DynamicsRNA Research and SplicingDNA Repair Mechanisms