Dimethyl Sulfoxide: A Bio-Friendly or Bio-Hazard Chemical? The Effect of DMSO in Human Fibroblast-like Synoviocytes
Manuel Gallardo‐Villagrán, Lucie Paulus, David Y. Léger, Bruno Therrien, Bertrand Liagre
Abstract
The effect of dimethyl sulfoxide (DMSO) in rheumatoid arthritis (RA) human fibroblast-like synoviocytes (FLSs) has been studied on five different samples harvested from the joints (fingers, hands and pelvis) of five women with RA. At high concentrations (>5%), the presence of DMSO induces the cleavage of caspase-3 and PARP-1, two phenomena associated with the cell death mechanism. Even at a 0.5% concentration of DMSO, MTT assays show a strong toxicity after 24 h exposure (≈25% cell death). Therefore, to ensure a minimum impact of DMSO on RA FLSs, our study shows that the concentration of DMSO has to be below 0.05% to be considered safe.
Topics & Concepts
Dimethyl sulfoxideFibroblastChemistryToxicityApoptosisCellViability assayProgrammed cell deathCleavage (geology)MTT assayBiochemistryBiologyOrganic chemistryIn vitroFracture (geology)PaleontologyRheumatoid Arthritis Research and TherapiesCardiac electrophysiology and arrhythmiasViral Infections and Immunology Research