Optimizing a Screening Protocol for Potential Extended-Spectrum β-Lactamase Escherichia coli on MacConkey Agar for Use in a Global Surveillance Program
Megan E. Jacob, Shivaramu Keelara, Awa Aïdara‐Kane, Jorge Raul Matheu Alvarez, Paula J. Fedorka–Cray
Abstract
The increasing prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli is worrisome. Coordinated efforts to better understand global prevalence and risk factors are needed. Developing lower- and middle-income countries need reliable, readily available, and cost-effective solutions for detecting ESBL E. coli to contribute to global surveillance. We evaluated MacConkey agar supplemented with ceftriaxone or cefotaxime as a screening method for accurately detecting and quantifying potential ESBL E. coli .
Topics & Concepts
MacConkey agarCefotaximeEscherichia coliProtocol (science)BiologyAgarMicrobiologyCeftriaxoneBeta-lactamaseMedicineAntibioticsBacteriaGeneticsPathologyAlternative medicineGeneAntibiotic Resistance in BacteriaAntibiotic Use and ResistanceVibrio bacteria research studies