Influence of Storage Conditions on SARS-CoV-2 Nucleic Acid Detection in Throat Swabs
Lin Li, Xiao Li, Zhendong Guo, Zhongyi Wang, Ke Zhang, Chao Li, Changjun Wang, Shoufeng Zhang
Abstract
The detection of SARS-CoV-2 infection is the premise of quarantine. In many countries or areas, samples need to be shipped or inactivated before SARS-CoV-2 testing. In this study, we checked the influence of sample storage conditions on SARS-CoV-2 nucleic acid testing results, including sample inactivation time, storage temperature, and storage time. All of these conditions caused an increase in the cycle threshold values of the nucleic acid tests and led to the misclassification of at least 10.2% of positive cases as negative or suspected. The results highlight the importance of immediate testing of samples for SARS-CoV-2 nucleic acid detection.
Topics & Concepts
Nucleic acidNucleic acid detectionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)ThroatVirologyCoronavirus disease 2019 (COVID-19)Nucleic acid testBiologyChemistryMedicineBiochemistryPathologyDiseaseAnatomyInfectious disease (medical specialty)SARS-CoV-2 detection and testingSARS-CoV-2 and COVID-19 ResearchCOVID-19 diagnosis using AI