Activation of apoptosis and G0/G1 cell cycle arrest along with inhibition of melanogenesis by humic acid and fulvic acid: <i>BAX/BCL</i>-2 and <i>Tyr</i> genes expression and evaluation of nanomechanical properties in A375 human melanoma cell line.
Mitra Salehi, Hossein Piri, Alireza Farasat, Babak Pakbin, Nematollah Gheibi
Abstract
Objectives: Humic acid (HA) and Fulvic acid (FA) are major members of humic substances, which are extracted from organic sources including soil and peat. The pro-apoptotic and anti-melanogenic effects of HA and FA at the cellular and molecular levels in the A375 human melanoma cell line were examined in this study. Materials and Methods: genes. Moreover, the changes in nanomechanical properties were determined through atomic force microscopy (AFM). Results: gene expression. Conclusion: gene. It is worthwhile to note that, HA and FA can be regarded to design new anti-cancer and anti-melanogenesis products.
Topics & Concepts
ApoptosisFlow cytometryViability assayCell cycleHumic acidFulvic acidChemistryMolecular biologyGene expressionCellCell cultureCell cycle checkpointGeneCell biologyBiologyBiochemistryGeneticsFertilizerOrganic chemistryHumic Substances and Bio-Organic Studies