PD-1 is expressed in cytotoxic granules of NK cells and rapidly mobilized to the cell membrane following recognition of tumor cells
Cecilia Pesini, Sandra Hidalgo, Maykel Arias, Llipsy Santiago, Carlota Calvo, Maitane Ocáriz-Díez, Dolores Isla, Pilar M. Lanuza, María José Agustín, Eva M. Gálvez, Ariel Ramírez-Labrada, Julián Pardo
Abstract
, which was partly reversed by using anti-PD-1 blocking antibodies. Our results indicate that NK cells from healthy individuals express cytotoxic granule-associated PD-1, which is rapidly mobilized to the cell membrane after interaction with tumor target cells. This novel finding helps to understand how PD-1 expression is regulated on NK cell membrane and the functional consequences of this expression during the elimination of tumor cells, which will help to design more efficient NK cell-based cancer immunotherapies.
Topics & Concepts
DegranulationCytotoxic T cellLymphokine-activated killer cellCell biologyCellInterleukin 21NK-92ChemistryCancer cellInterleukin 12BiologyIn vitroCancerReceptorBiochemistryGeneticsImmune Cell Function and InteractionCAR-T cell therapy researchCancer Immunotherapy and Biomarkers