A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition
Federico Pecori, Ikuko Yokota, Hisatoshi Hanamatsu, Taichi Miura, Chika Ogura, Hayato Ota, Jun‐ichi Furukawa, Shinya Oki, Kazuo Yamamoto, Osamu Yoshie, Shoko Nishihara
Abstract
Embryonic stem cells (ESCs) and epiblast-like cells (EpiLCs) recapitulate in vitro the epiblast first cell lineage decision, allowing characterization of the molecular mechanisms underlying pluripotent state transition. Here, we performed a comprehensive and comparative analysis of total glycomes of mouse ESCs and EpiLCs, revealing that overall glycosylation undergoes dramatic changes from early stages of development. Remarkably, we showed for the first time the presence of a developmentally regulated network orchestrating glycosylation changes and identified polycomb repressive complex 2 (PRC2) as a key component involved in this process. Collectively, our findings provide novel insights into the naïve-to-primed pluripotent state transition and advance the understanding of glycosylation complex regulation during early mouse embryonic development.