Development of a Targeted Gene Disruption System in the Poly(Ethylene Terephthalate)-Degrading Bacterium Ideonella sakaiensis and Its Applications to PETase and MHETase Genes
Shin-ichi Hachisuka, Tarou Nishii, Shosuke Yoshida
Abstract
The poly(ethylene terephthalate) (PET)-degrading bacterium Ideonella sakaiensis possesses two unique enzymes able to serve in PET hydrolysis. PET hydrolase (PETase) hydrolyzes PET into mono(2-hydroxyethyl) terephthalic acid (MHET) and MHET hydrolase (MHETase) hydrolyzes MHET into terephthalic acid (TPA) and ethylene glycol (EG).
Topics & Concepts
BacteriaGeneEthylenePoly ethyleneMicrobiologyBiologyChemistryBiotechnologyGeneticsBiochemistryCatalysisMicroplastics and Plastic Pollutionbiodegradable polymer synthesis and propertiesGraphene and Nanomaterials Applications