Post-synthetic benzylation of the mRNA 5′ cap <i>via</i> enzymatic cascade reactions
Nicolas V. Cornelissen, Raminta Mineikaitė, Mehmet Erguven, Nils Muthmann, A. Peters, Arthur Bartels, Andrea Rentmeister
Abstract
translation and to 128-150% in four different cell lines. This 5' cap modification did not activate cytosolic pathogen recognition receptors TLR3, TLR7 or TLR8 significantly more than control mRNAs, underlining its potential to contribute to the development of future mRNA therapeutics.
Topics & Concepts
Messenger RNATranslation (biology)Protein biosynthesisChemistryBiomoleculeReceptorEnzymeBiochemistryCell biologyBiologyGeneRNA Interference and Gene DeliveryVirus-based gene therapy researchRNA and protein synthesis mechanisms