Multi-omics-based label-free metabolic flux inference reveals obesity-associated dysregulatory mechanisms in liver glucose metabolism
Saori Uematsu, Satoshi Ohno, Kaori Tanaka, Atsushi Hatano, Toshiya Kokaji, Yuki Ito, Hiroyuki Kubota, Ken‐ichi Hironaka, Yutaka Suzuki, Masaki Matsumoto, Keiichi I. Nakayama, Akiyoshi Hirayama, Tomoyoshi Soga, Shinya Kuroda
Abstract
mice, we found that increased metabolic flux through gluconeogenesis resulted primarily from increased transcripts, whereas that through the pyruvate cycle resulted from both increased transcripts and changes in substrates of metabolic enzymes. With OMELET, we identified mechanisms underlying the obesity-associated dysregulation of metabolic flux in the liver.
Topics & Concepts
Metabolic flux analysisFlux (metallurgy)MetabolomicsMetabolic pathwayGlucose homeostasisGluconeogenesisTranscriptomeBiologyMetabolismMetabolomeHomeostasisEnzymeBiochemistryEnergy homeostasisCarbohydrate metabolismOmicsObesityChemistryBioinformaticsInsulin resistanceCell biologyEndocrinologyGene expressionGeneOrganic chemistryMicrobial Metabolic Engineering and BioproductionMetabolomics and Mass Spectrometry StudiesBioinformatics and Genomic Networks