Massilia soli sp. nov., isolated from soil
Ju Hye Baek, Woonhee Baek, Wenting Ruan, Hye Su Jung, Sung Chul Lee, Che Ok Jeon
Abstract
A Gram-stain-negative, catalase- and oxidase-positive and aerobic bacterium, designated strain R798 T , was isolated from soil in South Korea. Cells were motile rods by means of a single polar flagellum. Growth of strain R798 T was observed at 15–35 °C (optimum, 25–30 °C), pH 5.0–8.0 (optimum, 6.0) and 0–1.5 % NaCl (optimum, 0.3 %). Strain R798 T contained ubiquinone-8 as the sole isoprenoid quinone, summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ) and C 16 : 0 as the major fatty acids and phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The DNA G+C content of strain R798 T calculated from the whole genome sequence was 63.3 mol%. Phylogenetic analyses based on the 16S rRNA gene and whole genome sequences revealed that strain R798 T formed a distinct phyletic lineage within the genus Massilia . Strain R798 T was most closely related to Massilia eurypsychrophila B528-3 T with a 98.0 % 16S rRNA gene sequence similarity. Average nucleotide identity and digital DNA–DNA hybridization values between strain R798 T and the type strain of M. eurypsychrophila were 79.2 and 22.7 %, respectively. Based on the phenotypic, chemotaxonomic and molecular analyses, strain R798 T represents a novel species of the genus Massilia , for which the name Massilia soli sp. nov. is proposed. The type strain is R798 T (=KACC 22114 T =JCM 34601 T ).